Female Fertility Tests Explained — AMH, HSG, FSH, TSH and More | Dr. Dipesh Sorathiya
Dr. Dipesh Sorathiya
The most common mistake is waiting and hoping. For women over 35, time is a real factor. A fertility evaluation is not a dramatic step — it's simply information that helps you plan.

When Should Fertility Tests Be Done?

Your AgeWhen to See a Specialist
Under 35After 12 months of regular unprotected intercourse without pregnancy
35 and overAfter 6 months — don't wait a full year
40 and overBook a consultation before you start trying, or immediately
Any ageIf you have irregular periods, a known condition (PCOS, endometriosis, thyroid disease), or a previous miscarriage — earlier investigation is appropriate

Complete Female Fertility Workup — The Tests Explained

1. Ovarian Reserve Assessment — How Many Eggs Do You Have?

AMH (Anti-Müllerian Hormone)

  • Simple blood test — can be done on any day of the cycle
  • Measures the hormone produced by small follicles in the ovaries — gives an indication of the remaining egg pool
  • Low AMH = smaller reserve — time is more pressing
  • High AMH = large pool of follicles (often seen in PCOS)

AFC (Antral Follicle Count)

  • Done by transvaginal ultrasound on day 2–3 of the cycle
  • The doctor counts the small follicles visible in both ovaries
  • Directly reflects available egg reserve

FSH and LH (Day 2–3 Blood Test)

  • FSH above 10 mIU/mL suggests declining ovarian reserve
  • The LH:FSH ratio helps in diagnosing PCOS

2. Hormonal Panel

TestWhat It Checks
TSHThyroid function — critical for fertility and pregnancy
ProlactinElevated prolactin blocks ovulation
Testosterone, DHEASAndrogen levels — PCOS assessment
Fasting insulin + glucoseInsulin resistance
Estradiol (day 2–3)Baseline oestrogen level

3. Uterus and Fallopian Tube Assessment

Transvaginal Ultrasound (TVS) — The standard first-line imaging. Assesses:

  • Uterine shape, size, and lining
  • Ovaries and follicle count
  • Presence of fibroids, polyps, or cysts

HSG (Hysterosalpingography)

  • Done on day 7–10 of the cycle (after period, before ovulation)
  • An X-ray with contrast dye injected through the cervix — shows whether the fallopian tubes are open
  • If dye doesn't spill through the ends of the tubes — blockage suspected
  • This test is important and should not be skipped without good reason

3D Ultrasound or Saline Infusion Sonography (SIS) — Provides detailed assessment of the uterine cavity — useful for detecting polyps, a uterine septum, or submucosal fibroids.

Hysteroscopy — A camera inserted directly into the uterine cavity — the gold standard for evaluating the cavity. Can diagnose and treat in the same procedure.

Laparoscopy — Camera into the pelvis — identifies endometriosis, adhesions, and tube problems that other tests may miss.

4. Infectious Disease Screening

  • Rubella immunity: If not immune, vaccinate before conceiving
  • Thyroid antibodies: Autoimmune thyroid assessment
  • STI screening (Chlamydia particularly): Often silent, but can cause significant tube damage

5. Partner's Semen Analysis — Equally Important

Male factor accounts for 35–40% of infertility. Testing the woman comprehensively while skipping the male partner is only half a picture.

A semen analysis (count, motility, morphology) should always be done alongside the female workup. In many cases, adding DFI (DNA fragmentation) and CASA provides a much more complete picture.

When Should Each Test Be Done?

TestTimingReason
AMH, Prolactin, TSHAny day of cycleNot cycle-dependent
FSH, LH, Estradiol, AFCCycle day 2–3Baseline hormonal status
HSGCycle day 7–10After period, before ovulation
ProgesteroneDay 21 (in a 28-day cycle)Confirms ovulation occurred
Fasting glucose and insulinMorning fastingMetabolic assessment

Get a Clear Picture of Your Fertility

A structured fertility workup gives you information, not a verdict — and the earlier you have it, the more options you keep. Consult Dr. Dipesh Sorathiya at Khushhi IVF, Thaltej, Ahmedabad to plan the right tests for your situation.

Book an Appointment Learn About the Clinic

Numbers Are Not the Whole Story

The same AMH result of 0.8 ng/mL carries very different implications for a 30-year-old versus a 38-year-old. Numbers need to be interpreted in the context of your age, symptoms, your partner's results, and the clinical picture overall.

Do not try to interpret your fertility test results alone from an internet search. Bring them to your doctor and have a full conversation about what they mean for your specific situation.

Frequently Asked Questions

Does low AMH mean I can't get pregnant?

No. AMH is an indicator of ovarian reserve, not an absolute predictor of fertility. Many women with low AMH conceive — naturally or through IVF. What low AMH does mean is that time matters more for you, so acting sooner rather than later is wise.

Is an HSG painful?

Some women feel mild discomfort similar to period cramps. Others experience more significant pain. Your doctor can prescribe a painkiller before the procedure. It is brief — usually 5–10 minutes.

Do I need all these tests at once?

No. Your doctor will order tests based on your age, symptoms, and history. A structured, systematic approach is better than ordering everything simultaneously.

What does fertility testing cost in India?

AMH: approximately ₹1,500–3,000; HSG: ₹2,000–4,000; full hormonal panel: ₹3,000–6,000. Costs vary between clinics and cities.

Medical Disclaimer: This article is intended for educational purposes only and should not be considered a substitute for professional medical advice, diagnosis, or treatment. Fertility care should always be based on an individual medical evaluation by a qualified fertility specialist. Egg freezing procedures are subject to the applicable provisions of the Assisted Reproductive Technology (ART) Act, 2021, in India.

Khushhi IVF 5th Floor, Soham Pristine, Off Sindhubhavan Road, Thaltej, Ahmedabad, Gujarat 380054, India.
This article is for general informational purposes and is not a substitute for individual medical advice.